| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SERPINA12 recombinant protein (Position: K31-L376) was used as the immunogen for the SERPINA12 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SERPINA12 Antibody / Serpin A12 / Vaspin is a anti-SERPINA12 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SERPINA12
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
SERPINA12 inhibits proteases that degrade insulin-sensitizing factors and inflammatory mediators, contributing to glucose regulation and vascular protection. Experimental models demonstrate that Vaspin administration improves glucose tolerance and reduces insulin resistance in obese and diabetic subjects. Mechanistically, it modulates serine protease activity in adipose tissue and the liver, attenuating inflammatory cytokine production and endothelial dysfunction. It may also interact with GRP78 on cell surfaces to activate downstream signaling that promotes insulin sensitivity and cellular protection.
The SERPINA12 antibody is widely used in endocrinology, metabolism, and cardiovascular research to study the regulation and function of Vaspin. Western blot analysis typically identifies a 45 kilodalton band corresponding to the unglycosylated form of the protein, while immunohistochemistry shows cytoplasmic and extracellular staining in adipose and hepatic tissues. This antibody supports investigation of the molecular mechanisms underlying insulin resistance, obesity, and metabolic inflammation.
Beyond metabolic regulation, SERPINA12 is associated with cardiovascular protection through inhibition of vascular inflammation and endothelial apoptosis. It may also have roles in tissue repair and cancer progression.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
