| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human Sestrin 2/SESN2 recombinant protein (Position: I48-D341) was used as the immunogen for the SESN2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SESN2 Antibody / Sestrin 2 is a anti-SESN2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: SESN2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, FACS, ELISA
Biological background
Functionally, SESN2 antibody identifies a 480-amino-acid protein induced by p53, FOXO, and ATF4 transcription factors in response to stress stimuli such as DNA damage, hypoxia, and reactive oxygen species. SESN2 activates AMP-activated protein kinase (AMPK) and inhibits mammalian target of rapamycin complex 1 (mTORC1) by interacting with the GATOR2 complex, thereby reducing anabolic activity and promoting autophagy. It also regenerates overoxidized peroxiredoxins, restoring cellular antioxidant capacity and maintaining metabolic equilibrium.
The SESN2 gene is located on chromosome 1p35.3 and is expressed in metabolically active tissues such as liver, skeletal muscle, and heart. Its expression levels increase during fasting, exercise, or oxidative stress, making SESN2 a crucial component of the cellular adaptive stress response.
Pathologically, dysregulation of SESN2 contributes to aging, metabolic disorders, and cancer. Reduced SESN2 activity promotes oxidative stress, mitochondrial dysfunction, and insulin resistance, while its overexpression has protective effects against neurodegeneration and tissue injury. Research using SESN2 antibody supports studies in autophagy, metabolism, and stress signaling pathways.
SESN2 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect oxidative stress regulators.
Structurally, Sestrin 2 contains a conserved catalytic domain with oxidoreductase-like motifs, though it lacks enzymatic activity. Instead, its structure supports binding to GATOR complexes and peroxiredoxins. This antibody facilitates detailed investigation of SESN2's role in energy sensing, antioxidant defense, and lifespan regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
