| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SETBP1 recombinant protein (Position: S172-A1519) was used as the immunogen for the SETBP1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SETBP1 Antibody / SET binding protein 1 is a anti-SETBP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse.
Key elements and design rationale
- Target: SETBP1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
SETBP1 is encoded by the SETBP1 gene on human chromosome 18q12.3. The protein is approximately 170 kilodaltons and contains multiple AT-hook motifs for DNA binding, nuclear localization signals, and a SET-binding domain that mediates interaction with the SET protein, a known inhibitor of protein phosphatase 2A (PP2A). Through this interaction, SETBP1 indirectly modulates PP2A activity, influencing cell cycle progression and epigenetic control.
The SETBP1 antibody detects a 145-150 kilodalton band by western blot and shows nuclear localization under immunofluorescence. SETBP1 acts as a transcriptional regulator that binds GC-rich promoter regions to enhance expression of target genes, including HOXA cluster members associated with hematopoietic differentiation and leukemogenesis. Mutations that stabilize SETBP1 lead to aberrant accumulation, transcriptional reprogramming, and malignant transformation in myelodysplastic syndromes and acute myeloid leukemia.
In addition to cancer, germline mutations in SETBP1 cause Schinzel-Giedion syndrome, a severe developmental disorder characterized by craniofacial abnormalities and neurological impairment. These mutations increase SETBP1 stability, disrupt normal transcriptional repression, and impair neuronal differentiation. SETBP1 also interacts with chromatin-modifying complexes to coordinate histone acetylation and methylation status.
As a molecular bridge between SET and chromatin, SETBP1 integrates signaling pathways that regulate proliferation, apoptosis, and gene expression.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
