| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SETD5 recombinant protein (Position: Q196-R1275) was used as the immunogen for the SETD5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SETD5 Antibody / SET domain-containing protein 5 is a anti-SETD5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: SETD5
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Structurally, SETD5 is a large nuclear protein of approximately 1,442 amino acids and 170 kilodaltons containing an N-terminal SET domain and coiled-coil regions that mediate interactions with epigenetic cofactors such as HDAC3 and PAF1C. SETD5 localizes primarily to euchromatin, where it contributes to transcriptional repression and developmental gene regulation. It is broadly expressed, with highest levels in brain and embryonic tissues, supporting its essential role in differentiation and neurogenesis.
The SETD5 antibody is widely used in epigenetics, neuroscience, and developmental biology research to study chromatin remodeling, gene silencing, and neurodevelopmental disorders. Western blot analysis detects a 170 kilodalton band corresponding to SETD5, while immunofluorescence demonstrates nuclear localization with punctate chromatin-associated staining. This antibody provides a reliable reagent for assessing epigenetic enzyme expression and chromatin regulatory function.
Loss-of-function mutations in SETD5 are associated with autosomal dominant intellectual disability and developmental delay syndromes. Functional studies show that SETD5 regulates genes involved in cell cycle control, neural differentiation, and synapse formation through coordination of histone deacetylation and RNA polymerase II elongation. It acts as an integrator of epigenetic and transcriptional machinery, maintaining proper expression of developmental regulators. Dysregulated SETD5 expression has been linked to tumor progression, particularly in cancers with altered chromatin modification landscapes. The SETD5 antibody provides an essential tool for exploring the interplay between chromatin dynamics, transcriptional control, and development.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
