| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SETDB1 recombinant protein (Position: E20-E871) was used as the immunogen for the SETDB1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SETDB1 Antibody / Histone-lysine N-methyltransferase SETDB1 is a anti-SETDB1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: SETDB1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
SETDB1 contains a SET domain conferring methyltransferase activity, a methyl-CpG-binding domain (MBD), and a tudor domain that facilitate chromatin targeting. It forms complexes with co-repressors such as KAP1 (TRIM28) and HP1, directing H3K9 trimethylation at retroelements, developmental genes, and repetitive sequences. Through this mechanism, SETDB1 maintains transcriptional repression and contributes to X-chromosome inactivation, embryonic stem cell identity, and defense against endogenous retroviral elements.
The SETDB1 antibody is widely used in epigenetics, developmental biology, and cancer research to examine histone methylation and transcriptional silencing. Western blot analysis identifies a 170 kilodalton band corresponding to SETDB1, while immunofluorescence reveals punctate nuclear staining colocalizing with heterochromatin regions. This antibody enables researchers to assess chromatin states, epigenetic modifications, and silencing pathways across diverse cell types.
Aberrant SETDB1 expression and activity have been linked to multiple cancers, including melanoma, glioma, and lung carcinoma, where it promotes oncogenic transcriptional reprogramming and genomic instability. SETDB1 overexpression can also suppress immune responses by silencing interferon-stimulated genes. The SETDB1 antibody provides a precise reagent for studying epigenetic repression, gene regulation, and chromatin-based disease mechanisms.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
