| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived mouse SETX recombinant protein (Position: S28-R2447) was used as the immunogen for the SETX antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SETX Antibody / Senataxin is a anti-SETX Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Mouse, Rat.
Key elements and design rationale
- Target: SETX
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Senataxin interacts with transcription termination factors, RNA-processing proteins, and DNA repair complexes to integrate transcriptional and genomic maintenance pathways. It facilitates the repair of transcription-associated DNA damage and supports RNA splicing fidelity by ensuring timely R-loop resolution. SETX localizes to nuclear foci associated with active transcription sites and DNA damage response regions.
The SETX antibody is widely used in molecular genetics, neurobiology, and genome stability research to study helicase activity, R-loop metabolism, and transcriptional control. Western blot analysis identifies a 303 kilodalton band corresponding to Senataxin, while immunofluorescence reveals discrete nuclear foci marking transcriptionally active chromatin. This antibody supports investigations into RNA-DNA hybrid regulation and the prevention of replication stress.
Mutations in SETX cause neurological disorders such as ataxia with oculomotor apraxia type 2 (AOA2) and amyotrophic lateral sclerosis type 4 (ALS4), highlighting its role in maintaining neuronal integrity. Dysfunctional Senataxin results in R-loop accumulation, transcriptional stress, and DNA strand breaks. The SETX antibody provides a reliable reagent for exploring the links between transcription, RNA metabolism, and neurodegeneration.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
