| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SEZ6L2 recombinant protein (Position: E33-I910) was used as the immunogen for the SEZ6L2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SEZ6L2 Antibody / Seizure 6-like protein 2 is a anti-SEZ6L2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cell membrane, ER.
Key elements and design rationale
- Target: SEZ6L2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
SEZ6L2 is encoded by the SEZ6L2 gene located on human chromosome 16p11.2. The protein is approximately 922 amino acids long and contains three CUB domains and several complement control protein (CCP) repeats, which facilitate protein interactions in the extracellular environment. SEZ6L2 localizes to the neuronal plasma membrane and is enriched in dendritic and axonal compartments.
The SEZ6L2 antibody detects a 100 kilodalton band by western blot and reveals punctate synaptic staining in neuronal cultures. SEZ6L2 participates in the regulation of synaptic vesicle cycling, neuronal excitability, and calcium-dependent signaling. It also modulates the maturation of AMPA receptors, influencing excitatory synaptic transmission and plasticity. In the central nervous system, SEZ6L2 contributes to circuit development and synaptic pruning.
Mutations or deletions of SEZ6L2 have been linked to autism spectrum disorder, intellectual disability, and schizophrenia, reflecting its critical role in neural connectivity. Dysregulation of SEZ6L2 expression affects neuronal survival and synaptic balance, contributing to altered neurotransmission and cognitive dysfunction. It is also expressed in certain cancers, where it may promote cell adhesion and metastasis.
As a neuronal surface protein involved in communication and network formation, SEZ6L2 serves as a valuable target for studying synaptic biology and neurological disease mechanisms.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
