| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SFMBT2 recombinant protein (Position: Q136-D851) was used as the immunogen for the SFMBT2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SFMBT2 Antibody / Scm-like with four MBT domains protein 2 is a anti-SFMBT2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: SFMBT2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, IP, ELISA
Biological background
Functionally, SFMBT2 antibody identifies a 1220-amino-acid nuclear protein that binds to methylated histones, particularly histone H3 dimethylated on lysine 9 (H3K9me2) and lysine 27 (H3K27me2). SFMBT2 functions as a transcriptional corepressor by stabilizing repressive chromatin domains, thereby restricting transcriptional activation of developmental and cell cycle genes. It is a structural homolog of Drosophila Scm (Sex comb on midleg), which participates in Polycomb repressive complex formation.
The SFMBT2 gene is located on chromosome 8q13.3 and encodes a protein widely expressed in embryonic and adult tissues, with enrichment in stem cells and reproductive organs. SFMBT2 plays a key role in early embryogenesis and trophoblast differentiation by maintaining chromatin architecture and silencing specific developmental genes. In trophoblast stem cells, SFMBT2 contributes to maintenance of stemness and regulation of imprinted gene expression.
Epigenetically, SFMBT2 interacts with histone deacetylases (HDACs) and other repressive complexes to enforce gene silencing. It binds methylated histones through its MBT (malignant brain tumor) domains, which function as methyl-lysine recognition modules. Loss or depletion of SFMBT2 leads to de-repression of silenced loci, chromatin decondensation, and transcriptional misregulation. In cancer, altered SFMBT2 expression has been observed in hepatocellular carcinoma and prostate cancer, implicating it in tumor growth and chromatin remodeling.
SFMBT2 antibody is widely used in epigenetics, chromatin biology, and transcriptional regulation studies. It is suitable for immunoblotting, chromatin immunoprecipitation (ChIP), and immunofluorescence to investigate SFMBT2 localization and histone interactions. This antibody aids in exploring PcG-mediated gene repression and chromatin dynamics in development and disease. It also helps characterize the role of SFMBT2 in maintaining heterochromatin and transcriptional stability.
Structurally, SFMBT2 contains four MBT domains arranged in tandem that form a histone-binding scaffold, along with N-terminal coiled-coil motifs that mediate protein-protein interactions. Post-translational regulation includes phosphorylation and ubiquitination, which may influence chromatin association.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
