| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SFXN4 recombinant protein (Position: M1-V337) was used as the immunogen for the SFXN4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SFXN4 Antibody / Sideroflexin 4 is a anti-SFXN4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Mitochondria).
Key elements and design rationale
- Target: SFXN4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ICC/IF, FACS, ELISA
Biological background
Functionally, SFXN4 antibody identifies a 317-amino-acid transmembrane protein that contributes to the maturation of mitochondrial iron-sulfur (Fe-S) proteins. SFXN4 facilitates iron import into the mitochondrial matrix and interacts with key components of the Fe-S assembly machinery, including ISCU and NFS1. Through this interaction, it ensures the proper incorporation of Fe-S clusters into enzymes critical for electron transport, oxidative phosphorylation, and metabolic regulation. Disruption of SFXN4 impairs mitochondrial respiration, leading to decreased ATP production and increased oxidative stress.
The SFXN4 gene is located on chromosome 10q26.3 and encodes a protein with multiple transmembrane domains localized to the inner mitochondrial membrane. It belongs to the sideroflexin family, which includes five human homologs (SFXN1-SFXN5) with distinct but overlapping roles in amino acid and iron transport. Among these, SFXN4 has a specialized role in mitochondrial iron utilization and heme biosynthesis. Loss-of-function mutations in SFXN4 cause mitochondrial complex I and III deficiencies, resulting in mitochondrial myopathy, anemia, and developmental delay.
In normal physiology, SFXN4 maintains iron homeostasis and supports mitochondrial protein synthesis. It is essential for cell survival under metabolic stress conditions requiring efficient oxidative phosphorylation. Dysfunction of SFXN4 affects the activity of Fe-S cluster-dependent enzymes such as aconitase and succinate dehydrogenase, contributing to defects in the tricarboxylic acid (TCA) cycle and respiratory chain function.
SFXN4 antibody is widely used in mitochondrial biology, metabolism, and bioenergetics research. It is valuable for western blotting, immunofluorescence, and mitochondrial fractionation studies to analyze SFXN4 expression and localization. In disease research, this antibody helps investigate mitochondrial dysfunctions linked to anemia, neurodegeneration, and metabolic syndromes. Reduced SFXN4 expression is associated with impaired mitochondrial translation and decreased oxidative capacity, while overexpression may alter cellular redox balance.
Structurally, SFXN4 contains multiple hydrophobic helices forming a membrane-spanning topology that mediates metabolite and ion transport. Its function depends on coordination with mitochondrial carrier proteins and iron chaperones.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
