| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human Beta Sarcoglycan/SGCB recombinant protein (Position: E101-H318) was used as the immunogen for the SGCB antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SGCB Antibody / Beta Sarcoglycan is a anti-SGCB Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SGCB
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Beta sarcoglycan is a 318-amino acid membrane protein encoded on human chromosome 4q12. It contains a large extracellular domain with multiple N-glycosylation sites, a single transmembrane segment, and a short cytoplasmic tail. Proper assembly of the sarcoglycan complex is essential for maintaining membrane integrity during muscle contraction. Loss or misfolding of any component can destabilize the complex and lead to muscle fiber damage. Immunohistochemistry with SGCB antibody reveals strong sarcolemmal staining in normal skeletal and cardiac muscle, whereas dystrophic samples often show complete or partial absence of the protein.
The SGCB antibody is valuable for diagnostic and mechanistic studies of muscular dystrophies. In western blot, beta sarcoglycan is typically detected at 43�45 kDa. Its expression patterns provide insights into disease progression, compensatory mechanisms, and the effectiveness of gene replacement therapies. Research has shown that restoring SGCB expression in animal models can reassemble the DGC and improve muscle function, highlighting its potential as a therapeutic target. Moreover, beta sarcoglycan plays roles in cardiomyocyte adhesion and signal transduction, extending its relevance beyond skeletal muscle.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
