| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SGCZ recombinant protein (Position: M1-L245) was used as the immunogen for the SGCZ antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SGCZ Antibody / Sarcoglycan zeta is a anti-SGCZ Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SGCZ
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, SGCZ antibody identifies a 243-amino-acid single-pass membrane protein primarily expressed in skeletal and cardiac muscle. SGCZ associates with alpha-, beta-, gamma-, and delta-sarcoglycans to form a heterotetrameric complex that links dystrophin to the sarcolemma. This interaction is critical for maintaining membrane integrity during muscle contraction and relaxation cycles. SGCZ also participates in intracellular signaling that regulates calcium handling and muscle fiber repair.
The SGCZ gene is located on chromosome 8p22 and is expressed in skeletal muscle, heart, and smooth muscle tissues. Its transcription is regulated by myogenic factors including MYOD1 and MEF2, aligning expression with muscle differentiation and regeneration. SGCZ contributes to muscle architecture by connecting the cytoskeleton to the extracellular matrix through dystrophin and laminin interactions.
Pathologically, mutations in SGCZ cause limb-girdle muscular dystrophy type 2C-related phenotypes and contribute to sarcoglycanopathy spectrum disorders. Deficiency leads to sarcolemma fragility, fiber necrosis, and progressive muscle weakness. Reduced expression has also been observed in cardiomyopathies, where it contributes to cardiac dysfunction. Research using SGCZ antibody supports studies in muscle disease, sarcolemma structure, and dystrophin complex biology.
SGCZ antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect membrane glycoproteins in muscle tissue.
Structurally, Sarcoglycan zeta contains an extracellular glycosylated domain and a short cytoplasmic tail anchoring it to the dystrophin-associated complex. It interacts directly with beta- and delta-sarcoglycans and indirectly stabilizes dystrophin. This antibody facilitates exploration of SGCZ's role in muscle membrane integrity, mechanical signaling, and dystrophic pathology.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
