| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SH2B1 recombinant protein (Position: W26-Q715) was used as the immunogen for the SH2B1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SH2B1 Antibody / SH2B adapter protein 1 is a anti-SH2B1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SH2B1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Structurally, SH2B1 is a 756-amino-acid protein of approximately 85 kilodaltons containing an N-terminal dimerization domain, a central pleckstrin homology (PH) domain, and a C-terminal Src homology 2 (SH2) domain that mediates phosphotyrosine-dependent interactions. It exists in multiple isoforms (alpha, beta, gamma, and delta) produced by alternative splicing, with tissue-specific expression across brain, liver, adipose tissue, and skeletal muscle. The protein acts as a scaffold that bridges receptor kinases to intracellular effectors, amplifying or modulating signaling intensity and duration.
The SH2B1 antibody is widely used in metabolism, endocrinology, and neurobiology research to study insulin signaling, leptin sensitivity, and receptor-mediated pathways regulating energy homeostasis. Western blot analysis detects a band of approximately 85 kilodaltons corresponding to SH2B1, while immunofluorescence reveals cytoplasmic and plasma membrane localization. This antibody is an essential reagent for exploring the molecular basis of metabolic signaling and neuronal regulation.
Functionally, SH2B1 binds to insulin and leptin receptors through JAK2 and IRS1, enhancing phosphorylation cascades that regulate glucose uptake and appetite control. Loss or mutation of SH2B1 disrupts these pathways, leading to insulin resistance, obesity, and metabolic syndrome. Deletions encompassing SH2B1 are associated with severe early-onset obesity and neurobehavioral abnormalities, while overexpression can increase leptin sensitivity and improve glucose tolerance. Beyond metabolism, SH2B1 contributes to neuronal growth, regeneration, and synaptic plasticity through its modulation of NGF and BDNF signaling. The SH2B1 antibody provides a robust tool for studying these signaling mechanisms and their implications in metabolic and neurological disorders.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
