| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SHANK1 recombinant protein (Position: D66-R2161) was used as the immunogen for the SHANK1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SHANK1 Antibody / SH3 and multiple ankyrin repeat domains protein 1 is a anti-SHANK1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: SHANK1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
SHANK1 is encoded by the SHANK1 gene located on human chromosome 19q13.33. The protein exceeds 200 kDa and contains several structural domains: multiple ankyrin repeats, an SH3 domain, a PDZ domain, a proline-rich region that interacts with cortactin and Homer, and a sterile alpha motif (SAM) that mediates self-association. These domains enable SHANK1 to function as a scaffold linking glutamate receptors, adhesion molecules, and signaling enzymes into large PSD assemblies. In neurons, SHANK1 stabilizes AMPA and NMDA receptor complexes and regulates dendritic spine morphology.
The SHANK1 antibody is commonly used to label postsynaptic structures, showing punctate localization in dendritic spines consistent with PSD distribution. Western blot analysis typically reveals a prominent band between 240-260 kDa. Functional studies demonstrate that SHANK1 promotes synapse maturation and coordinates actin cytoskeleton dynamics, thereby influencing neuronal connectivity and excitatory signaling. Mutations or deletions of SHANK family genes are associated with autism spectrum disorder and intellectual disability, underscoring the functional importance of these scaffolding proteins in brain development.
Beyond the nervous system, SHANK1 expression has been observed in testis and other tissues where it may regulate cytoskeletal organization. Interaction partners include PSD-95, GKAP, Homer, and cortactin, forming a structural network essential for synaptic signal transduction. Loss of SHANK1 disrupts synaptic transmission and reduces spine density, while overexpression alters synaptic scaling and excitatory balance.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
