| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SHANK2 recombinant protein (Position: R845-D1827) was used as the immunogen for the SHANK2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SHANK2 Antibody / SH3 and multiple ankyrin repeat domains protein 2 is a anti-SHANK2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SHANK2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, SHANK2 antibody recognizes a synaptic scaffolding protein of approximately 180-200 kDa that coordinates synaptic architecture and signaling. SHANK2 interacts with PSD-95, Homer, and cortactin, linking surface glutamate receptors to the actin cytoskeleton. These interactions stabilize dendritic spine morphology and facilitate efficient synaptic transmission. SHANK2's PDZ, SAM, and ankyrin repeat domains enable it to integrate diverse protein-protein interactions, forming large macromolecular complexes essential for signal transduction.
The SHANK2 gene is located on chromosome 11q13.3 and encodes multiple isoforms through alternative splicing. SHANK2 is particularly abundant in the cerebral cortex, hippocampus, and cerebellum, where it regulates synaptic plasticity and neuronal connectivity. Genetic studies associate SHANK2 mutations and deletions with autism spectrum disorder (ASD), intellectual disability, and schizophrenia, linking its dysfunction to altered excitatory signaling and neural circuit development.
SHANK2 antibody is a powerful tool for neuroscience research, enabling investigation of postsynaptic organization, receptor clustering, and synaptic remodeling. It is widely applied in western blotting, immunofluorescence, and co-immunoprecipitation to study excitatory synapses and protein interactions within the PSD. In neuronal cultures, SHANK2 colocalizes with synaptic markers such as PSD-95 and Synapsin, marking mature excitatory synapses. Loss of SHANK2 expression disrupts dendritic spine density and reduces AMPA and NMDA receptor-mediated currents, impairing synaptic strength.
Structurally, SHANK2's N-terminal ankyrin repeats mediate cytoskeletal binding, while its C-terminal SAM domain enables oligomerization, forming mesh-like scaffolds beneath the postsynaptic membrane. Post-translational modifications, including phosphorylation and palmitoylation, regulate its localization and synaptic activity. SHANK2 also interacts with neuroligins and mGluR5, connecting receptor signaling to structural reorganization. Dysregulation of SHANK2 leads to impaired synaptic signaling and behavioral abnormalities in animal models.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
