| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SHARPIN recombinant protein (Position: H25-R325) was used as the immunogen for the SHARPIN antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SHARPIN Antibody / SHANK-associated RH domain-interacting protein is a anti-SHARPIN Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: SHARPIN
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
Functionally, SHARPIN antibody recognizes a 387-amino-acid protein that acts as a scaffolding subunit linking the ubiquitin ligase HOIP (RNF31) and HOIL-1L (RBCK1) to form the active LUBAC complex. This complex catalyzes linear (M1-linked) polyubiquitination of signaling proteins, stabilizing TNF receptor-associated complexes and promoting NF-kappaB activation. Through this pathway, SHARPIN regulates innate and adaptive immune responses, cell survival, and inflammatory gene transcription. It also negatively regulates apoptotic signaling by inhibiting caspase activation downstream of TNF receptors.
The SHARPIN gene is located on chromosome 8q24.3 and encodes a widely expressed cytoplasmic protein enriched in immune cells, skin, and neural tissues. Beyond immune regulation, SHARPIN interacts with integrins and actin-associated proteins to control cell adhesion and motility. It binds to the SHANK family of postsynaptic scaffolds, linking cytoskeletal dynamics to synaptic stability and plasticity. SHARPIN thus functions as both an immune modulator and structural adaptor in distinct cellular contexts.
Deficiency or mutation of SHARPIN disrupts LUBAC assembly, leading to impaired NF-kappaB signaling and increased apoptosis. Mouse models lacking SHARPIN exhibit severe chronic inflammation and skin lesions, known as the chronic proliferative dermatitis (cpdm) phenotype. In humans, SHARPIN dysregulation has been implicated in inflammatory diseases, cancer, and neurodegeneration. Elevated SHARPIN expression correlates with tumor progression in prostate and breast cancers, where it enhances PI3K/AKT signaling and resistance to apoptosis.
SHARPIN antibody is widely used for research into inflammatory signaling, ubiquitination, and immune regulation. Applications include immunoblotting, immunohistochemistry, and co-immunoprecipitation to analyze LUBAC complex formation and function. Its detection provides insight into TNF receptor signaling, cell death control, and integrin-mediated adhesion. In neurobiology, SHARPIN interacts with SHANK scaffolds and PSD-95 at excitatory synapses, influencing postsynaptic organization and neuronal communication.
Structurally, SHARPIN contains an N-terminal PH domain-like motif, a central ubiquitin-like (UBL) domain, and a C-terminal Npl4 zinc finger (NZF) domain responsible for binding linear ubiquitin chains. Post-translational regulation includes phosphorylation and ubiquitination, controlling its participation in signaling networks.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
