| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human SHB was used as the immunogen for the SHB antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SHB Antibody / SH2 domain-containing adapter protein B is a anti-SHB Rabbit antibody Recombinant Rabbit Monoclonal clone 31S61 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SHB
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31S61, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
SHB antibody is widely used in studies of angiogenesis, immune regulation, and cancer. SHB is known to associate with vascular endothelial growth factor receptor signaling and contributes to endothelial cell migration, survival, and sprouting. In immune cells, SHB modulates T cell receptor signaling and influences activation, proliferation, and cytokine production. Altered expression of SHB has been associated with tumor development and progression, underscoring its importance as both a research target and potential biomarker.
The antibody is suitable for western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. In western blot assays, SHB antibody detects protein bands of expected size, confirming its expression under different cellular conditions. Immunohistochemistry provides tissue distribution patterns, often highlighting expression in vascular structures and immune infiltrates. Immunofluorescence allows colocalization with kinases and receptors, demonstrating its adaptor role within signaling complexes. These applications make SHB antibody a versatile reagent for cell biology and disease research.
SHB has been implicated in diabetes related vascular complications, where it affects insulin signaling and endothelial function. By employing SHB antibody, researchers can explore how adaptor proteins influence metabolic and vascular health. In cancer biology, SHB links growth factor receptor signaling to pathways that drive tumor angiogenesis and invasion. Detecting SHB expression can therefore provide insight into tumor progression and therapy resistance.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
