| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SHD recombinant protein (Position: M1-P340) was used as the immunogen for the SHD antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SHD Antibody / SH2 domain-containing adapter protein D is a anti-SHD Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic.
Key elements and design rationale
- Target: SHD
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, FACS, ELISA
Biological background
Structurally, SHD is a 440-amino-acid protein of approximately 48 kilodaltons featuring a central SH2 domain flanked by low-complexity regions that facilitate interaction with membrane receptors and signaling complexes. It localizes to the cytoplasm and plasma membrane following receptor activation and participates in pathways initiated by growth factors, cytokines, and immune receptors. Through its SH2-mediated binding, SHD recruits kinases and phosphatases that regulate phosphorylation dynamics and cellular response outcomes.
The SHD antibody is widely used in immunology, signal transduction, and neurobiology research to study SH2 domain-mediated signaling, receptor cross-talk, and cytoplasmic adaptor functions. Western blot analysis detects a 48 kilodalton band corresponding to SHD, while immunofluorescence shows diffuse cytoplasmic and membrane-associated staining. This antibody enables detailed examination of signaling network architecture and adaptor-mediated pathway modulation.
Functionally, SHD contributes to the organization of signaling complexes downstream of tyrosine kinases, facilitating rapid response to extracellular cues. It modulates pathways involving MAPK, JAK/STAT, and PI3K signaling cascades, thereby influencing immune activation, synaptic development, and cell fate determination. Dysregulation of SHD expression may alter immune receptor signaling and neuronal differentiation, implicating the protein in both immunological and developmental disorders. The SHD antibody provides a valuable research tool for characterizing intracellular adaptor networks and phosphotyrosine-dependent signaling mechanisms.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
