| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SHE recombinant protein (Position: L36-H495) was used as the immunogen for the SHE antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SHE Antibody / SH2 domain-containing adapter protein E is a anti-SHE Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: SHE
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ICC/IF, FACS, ELISA
Biological background
SHE contains a central SH2 domain that binds phosphotyrosine motifs on activated receptors and associated kinases. Through its modular architecture, it acts as a scaffold that brings kinases and transcriptional regulators into close proximity, ensuring precise and timely propagation of receptor-initiated signals. SHE interacts with proteins such as EGFR, JAK2, and STAT3, influencing processes from hematopoietic differentiation to epithelial cell proliferation.
The SHE antibody is widely used in cell signaling, oncology, and hematology research to study adaptor-mediated signal transduction and receptor dynamics. Western blot analysis identifies a 62 kilodalton band corresponding to SHE, while immunofluorescence shows cytoplasmic and perinuclear localization in stimulated cells. This antibody is essential for dissecting receptor coupling events and understanding how adaptor proteins coordinate cellular responses to growth factors.
In cancer and immune regulation, altered SHE expression or phosphorylation disrupts normal signal transduction, leading to abnormal cell proliferation and survival. Overactivation of SHE-associated signaling contributes to tumor progression, while its suppression can impair cytokine responsiveness and differentiation. The SHE antibody provides a sensitive tool for evaluating these effects and monitoring adaptor function in normal and disease contexts.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
