| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SHMT2 recombinant protein (Position: A9-H504) was used as the immunogen for the SHMT2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SHMT2 Antibody / Serine hydroxymethyltransferase is a anti-SHMT2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: SHMT2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ICC/IF, IP, FACS, ELISA
Biological background
Serine hydroxymethyltransferase, mitochondrial forms homotetramers that utilize pyridoxal phosphate (PLP) as a cofactor for catalysis. Its activity links amino acid metabolism with folate-mediated one-carbon flux, coordinating mitochondrial and cytosolic metabolic networks. The SHMT2 antibody enables visualization of this enzyme within mitochondria, supporting studies of its spatial regulation and contribution to biosynthetic pathways under nutrient limitation or hypoxic conditions. SHMT2 expression increases in rapidly dividing cells to sustain nucleotide production and redox balance.
Aberrant activation of one-carbon metabolism, including SHMT2 upregulation, is a hallmark of cancer metabolism. Overexpression of Serine hydroxymethyltransferase, mitochondrial enhances proliferation and resistance to oxidative stress by supplying reduced folate cofactors and NADPH. The SHMT2 antibody facilitates detection of this metabolic enzyme in tumor tissues and cell lines, aiding evaluation of its role in tumorigenesis and metabolic adaptation. SHMT2 has been proposed as a potential therapeutic target, as inhibition disrupts nucleotide synthesis and redox homeostasis in cancer cells.
Beyond oncology, Serine hydroxymethyltransferase, mitochondrial contributes to neural development, mitochondrial respiration, and cellular methylation capacity. It provides one-carbon units necessary for formate generation and DNA methylation reactions that influence epigenetic regulation. The SHMT2 antibody supports research into these metabolic control points, enabling functional analysis of folate cycle dynamics in metabolic disorders, neurodegenerative diseases, and aging.
The SHMT2 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing clear mitochondrial staining patterns.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
