| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SIGIRR recombinant protein (Position: M1-E353) was used as the immunogen for the SIGIRR antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SIGIRR Antibody / Single Ig IL-1-related receptor is a anti-SIGIRR Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: SIGIRR
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ICC/IF, FACS, ELISA
Biological background
Single immunoglobulin and toll-interleukin 1 receptor domain-containing protein is a type I transmembrane glycoprotein with a single extracellular immunoglobulin-like domain, a transmembrane segment, and an intracellular TIR domain. Unlike classical toll-like receptors, its cytoplasmic TIR domain lacks certain signaling motifs, rendering it inhibitory. The SIGIRR antibody enables detection of this regulatory receptor in epithelial, endothelial, and immune cells, allowing studies on how it fine-tunes inflammatory signaling.
Functionally, SIGIRR interacts with IL-1 receptor and TLR complexes to disrupt adaptor recruitment and downstream kinase activation. It thereby attenuates NF-?B and MAPK pathway activation in response to cytokine or pathogen-associated stimuli. The SIGIRR antibody supports investigations into these signaling mechanisms and helps define how reduced SIGIRR expression contributes to hyperinflammatory states and autoimmune diseases. Mice deficient in this protein exhibit enhanced inflammatory responses and are more susceptible to colitis and sepsis, underscoring its protective role.
Beyond inflammation, Single immunoglobulin and toll-interleukin 1 receptor domain-containing protein contributes to mucosal homeostasis, microbiota balance, and epithelial integrity. In the gut and lungs, it limits IL-1-driven inflammation and maintains tolerance to commensal organisms. The SIGIRR antibody is used in immunohistochemistry and flow cytometry to evaluate tissue distribution, revealing strong expression on epithelial cells of mucosal surfaces. Aberrant regulation of SIGIRR has been linked to inflammatory bowel disease, asthma, and cancer, where loss of this inhibitory signaling may favor tumor-promoting inflammation.
The SIGIRR antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry. It provides clear membrane and cytoplasmic staining consistent with receptor localization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
