| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SIGLEC15 recombinant protein (Position: R22-R319) was used as the immunogen for the SIGLEC15 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SIGLEC15 Antibody / Sialic acid-binding Ig-like lectin 15 is a anti-SIGLEC15 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: SIGLEC15
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
Sialic acid-binding Ig-like lectin 15 is encoded by the SIGLEC15 gene located on human chromosome 18q12.3. The protein consists of an extracellular N-terminal V-set immunoglobulin domain that recognizes sialylated glycans, a transmembrane segment, and a short cytoplasmic tail that associates with adaptor proteins such as DAP12 or DAP10 to mediate downstream signaling. In osteoclasts, SIGLEC15 promotes differentiation and bone resorption by activating phosphoinositide 3-kinase and mitogen-activated protein kinase cascades. In immune cells, it functions as a checkpoint ligand that suppresses T-cell activation, analogous to PD-L1.
Studies using the SIGLEC15 antibody have shown that this protein is upregulated on tumor-associated macrophages and osteoclasts in various malignancies and inflammatory diseases. Western blot analyses typically detect a 35-40 kilodalton glycoprotein band. Immunohistochemistry demonstrates strong membrane staining in macrophage-rich regions of tumors and in bone-resorptive zones. Functionally, SIGLEC15 acts as an immune suppressor by binding unidentified receptors on T cells and natural killer cells, inhibiting cytokine production and cytotoxic responses. Its expression inversely correlates with PD-L1, suggesting a complementary role in immune evasion mechanisms exploited by tumors.
Therapeutic targeting of SIGLEC15 has emerged as a promising strategy in cancer immunotherapy, particularly for patients unresponsive to PD-1/PD-L1 blockade. The protein also regulates osteoclast activity and bone remodeling, making it relevant in osteoporosis and arthritis research.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
