| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human Snf1lk/SIK1 recombinant protein (Position: M1-Q783) was used as the immunogen for the SIK1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SIK1 Antibody / Salt-inducible kinase 1 is a anti-SIK1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: SIK1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
SIK1 is encoded by the SIK1 gene located on human chromosome 21q22.3. The protein is approximately 78 kilodaltons and consists of an N-terminal kinase domain, an autoinhibitory sequence, and a C-terminal region rich in phosphorylation sites. SIK1 modulates transcription by phosphorylating CREB-regulated transcription coactivators (CRTCs), leading to their cytoplasmic retention and suppression of CREB-dependent gene expression.
The SIK1 antibody detects a strong 85 kilodalton band by western blot. Under stress or hormonal stimulation, SIK1 undergoes rapid induction and nuclear translocation. It acts as a feedback regulator controlling CREB activity, gluconeogenic gene expression, and circadian rhythm stability.
Functionally, SIK1 plays an important role in metabolic homeostasis. It regulates hepatic glucose output, lipid metabolism, and skeletal muscle adaptation to exercise. In the brain, SIK1 contributes to neuronal plasticity and sleep-wake regulation. Dysregulation of SIK1 expression or phosphorylation has been implicated in hypertension, metabolic syndrome, and mood disorders.
SIK1 is a key mediator of hormonal signaling via cAMP and glucocorticoids. It interacts with 14-3-3 proteins and phosphatases, allowing dynamic control of kinase activity in response to changing energy states.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
