| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SIK2 recombinant protein (Position: Q278-H919) was used as the immunogen for the SIK2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SIK2 Antibody / Salt-inducible kinase 2 is a anti-SIK2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (ER), Nucleus.
Key elements and design rationale
- Target: SIK2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ICC/IF, FACS, ELISA
Biological background
Functionally, SIK2 antibody identifies a 926-amino-acid cytoplasmic protein containing an N-terminal kinase domain, an autoinhibitory region, and regulatory phosphorylation sites. SIK2 phosphorylates transcriptional coactivators such as CRTC2 and HDAC4, repressing cAMP-response element-binding protein (CREB)-mediated transcription. Through this action, SIK2 coordinates the response to metabolic hormones and nutrient availability, ensuring energy balance under varying physiological conditions.
The SIK2 gene is located on chromosome 11q23.3 and is expressed in adipose tissue, skeletal muscle, and brain. It plays a critical role in insulin signaling by modulating the expression of gluconeogenic and lipogenic enzymes. In adipocytes, SIK2 regulates hormone-sensitive lipase activity and lipid mobilization. It also influences mitochondrial biogenesis and oxidative metabolism, linking it to overall energy homeostasis. In the nervous system, SIK2 participates in neuronal differentiation and synaptic plasticity through phosphorylation of CREB cofactors.
In the cell cycle, SIK2 acts as a mitotic regulator. It localizes to centrosomes and controls spindle orientation and cytokinesis. Overexpression of SIK2 promotes centrosome separation and mitotic progression, while its inhibition disrupts mitosis and sensitizes cancer cells to chemotherapeutic agents. Elevated SIK2 expression has been reported in ovarian and breast cancers, where it supports cell proliferation and metabolic adaptation.
SIK2 antibody is widely used in studies of metabolism, kinase signaling, and cancer biology. It is suitable for western blotting, immunofluorescence, and kinase assay applications to detect endogenous SIK2 and phosphorylated substrates. In metabolic research, this antibody aids in evaluating AMPK-related signaling, energy regulation, and hormonal control of gene expression. In oncology, SIK2 serves as a potential therapeutic target due to its dual role in metabolism and mitotic control.
Structurally, SIK2 contains a conserved activation loop that undergoes phosphorylation by upstream kinases such as LKB1, essential for catalytic activation. Its activity is negatively regulated by cAMP-dependent protein kinase (PKA)-mediated phosphorylation, which induces 14-3-3 protein binding and cytoplasmic sequestration.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
