| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SIN3B recombinant protein (Position: Q127-E1127) was used as the immunogen for the SIN3B antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SIN3B Antibody / Paired amphipathic helix protein Sin3b is a anti-SIN3B Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: SIN3B
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ICC/IF, FACS, ELISA
Biological background
SIN3B is encoded by the SIN3B gene on human chromosome 19p13.11. The protein is approximately 150 kilodaltons and contains multiple paired amphipathic helix (PAH) domains that mediate interactions with transcription factors and chromatin-modifying enzymes. SIN3B serves as a scaffold that assembles HDAC1, HDAC2, RBBP4/7, and other corepressors into multiprotein complexes targeting specific promoters.
The SIN3B antibody detects a 150 kilodalton band by western blot and shows nuclear localization by immunofluorescence. Through recruitment of HDAC complexes, SIN3B deacetylates histones H3 and H4, resulting in chromatin compaction and transcriptional repression. It also interacts with transcription factors such as E2F4, MAD, and REST, enabling context-dependent regulation of genes involved in proliferation, senescence, and neuronal development.
SIN3B plays key roles in differentiation, acting as a switch from proliferation to quiescence. Knockdown of SIN3B leads to uncontrolled growth and defective chromatin silencing, while overexpression suppresses oncogenic transcription programs. Altered SIN3B function has been implicated in cancer, diabetes, and neurodevelopmental disorders due to disrupted epigenetic control.
As a master regulator of chromatin remodeling, SIN3B integrates multiple signaling pathways to maintain transcriptional balance.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
