| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SIPA1 recombinant protein (Position: D21-L1034) was used as the immunogen for the SIPA1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SIPA1 Antibody / Signal-induced proliferation-associated 1 is a anti-SIPA1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, Perinuclear.
Key elements and design rationale
- Target: SIPA1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
Structurally, SIPA1 contains an N-terminal RapGAP domain responsible for GTPase activation, a PDZ domain that mediates protein-protein interactions, and a C-terminal coiled-coil domain that regulates subcellular localization. It is predominantly localized to the cytoplasm and nucleus, where it integrates extracellular growth and adhesion cues with downstream transcriptional and cytoskeletal responses. SIPA1 interacts with integrins and focal adhesion components, influencing adhesion turnover and migration.
The SIPA1 antibody is widely used in cell signaling, oncology, and vascular biology research to study Rap GTPase regulation, adhesion signaling, and cancer cell dissemination. Western blot analysis detects a 120 kilodalton band corresponding to SIPA1, while immunofluorescence shows cytoplasmic and nuclear staining in epithelial and immune cells. This antibody enables characterization of Rap1 deactivation and adhesion dynamics under physiological and pathological conditions.
Functionally, SIPA1 acts as a metastasis suppressor by downregulating integrin signaling and preventing excessive adhesion and proliferation. Reduced expression or loss of SIPA1 disrupts Rap1 regulation, leading to enhanced cell spreading, migration, and metastasis formation in several cancers. In immune cells, SIPA1 modulates adhesion to vascular endothelium and migration across tissue barriers, linking Rap signaling to immune surveillance and inflammation. The SIPA1 antibody supports studies aimed at understanding how RapGAP activity integrates with cytoskeletal and transcriptional control mechanisms.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
