| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SIRT6 recombinant protein (Position: D14-S355) was used as the immunogen for the SIRT6 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SIRT6 Antibody / Sirtuin 6 is a anti-SIRT6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human. Reported localization: Nuclear, ER (minor).
Key elements and design rationale
- Target: SIRT6
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IF, ICC, WB
Biological background
Functionally, SIRT6 antibody recognizes a chromatin-associated protein that deacetylates histone H3 at lysine 9 and lysine 56, maintaining telomere integrity and DNA repair efficiency. SIRT6 also promotes base excision repair and regulates transcription by modulating chromatin compaction. It interacts with PARP1 and SNF2H to coordinate double-strand break repair and suppress genomic instability. Beyond chromatin regulation, SIRT6 controls glucose and lipid metabolism by repressing glycolytic gene expression and enhancing mitochondrial respiration.
The SIRT6 gene, located on chromosome 19p13.3, encodes a 355-amino-acid enzyme localized predominantly in the nucleus. SIRT6 expression is enriched in metabolically active tissues such as heart, liver, and brain. It modulates aging-related pathways by influencing insulin sensitivity, inflammation, and DNA repair. Deficiency of SIRT6 in mice causes premature aging, metabolic dysregulation, and shortened lifespan.
Pathologically, dysregulated SIRT6 activity has been implicated in cancer, diabetes, cardiovascular disease, and neurodegeneration. In cancer, SIRT6 may act as a tumor suppressor by limiting glycolysis and maintaining genomic integrity. In metabolic disorders, altered SIRT6 function disrupts energy homeostasis and inflammatory signaling. Research using SIRT6 antibody supports studies of chromatin remodeling, aging, and metabolic control.
SIRT6 antibody is validated for use in its applications, and it is widely applied in DNA repair and transcriptional regulation studies.
Structurally, SIRT6 contains a conserved Rossmann-fold domain responsible for NAD+ binding and a zinc-binding module stabilizing its catalytic core. The enzyme exhibits both deacetylase and long-chain deacylase activity, extending its regulatory range to lipid-modified substrates. This antibody aids in investigating SIRT6�s central role in chromatin biology and metabolic regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
