| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human C13orf3/SKA3 recombinant protein (Position: M1-N412) was used as the immunogen for the SKA3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SKA3 Antibody / Spindle and kinetochore associated complex subunit 3 / C13orf3 is a anti-SKA3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: SKA3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ELISA
Biological background
Functionally, SKA3 antibody identifies a 412-amino-acid cytoplasmic and kinetochore-localized protein that binds microtubules and interacts with the Ndc80 complex to anchor chromosomes to spindle fibers. The SKA complex is recruited to kinetochores in a microtubule-dependent manner and facilitates the coupling of depolymerizing microtubules to chromosome movement. SKA3 plays a crucial role in maintaining mitotic fidelity and preventing chromosome missegregation. It is also required for silencing the spindle assembly checkpoint once proper attachment is achieved, allowing progression into anaphase.
The SKA3 gene is located on chromosome 13q12.13 and is expressed in proliferating cells, with elevated expression during the G2/M phase of the cell cycle. Its transcription is regulated by E2F family transcription factors, linking SKA3 expression to cell cycle progression and mitotic entry.
Pathologically, dysregulation of SKA3 contributes to chromosomal instability, aneuploidy, and cancer. Overexpression has been observed in breast, liver, and lung cancers, where it promotes uncontrolled proliferation and correlates with poor prognosis. Loss of SKA3 function disrupts kinetochore attachment and induces mitotic arrest. Research using SKA3 antibody supports studies in mitosis, spindle checkpoint control, and cancer biology.
SKA3 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect mitotic spindle-associated proteins.
Structurally, Spindle and kinetochore associated complex subunit 3 contains coiled-coil domains that mediate oligomerization and interaction with SKA1/SKA2. Its C-terminal region binds directly to microtubules, while its N-terminal domain interacts with kinetochore complexes. This modular organization enables SKA3 to function as a mechanical linker between depolymerizing microtubules and kinetochores. This antibody allows precise analysis of SKA3's role in mitotic regulation and chromosomal stability.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
