| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human UT-B/SLC14A1 recombinant protein (Position: M1-L389) was used as the immunogen for the SLC14A1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SLC14A1 Antibody / Urea transporter 1 / UT-B is a anti-SLC14A1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Immunohistochemistry (IHC), Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SLC14A1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, IHC, WB, FACS
Biological background
Urea transporter 1 is highly expressed in erythrocytes, renal descending vasa recta, and bladder epithelium, where it regulates urea permeability and water reabsorption. The protein forms a transmembrane channel composed of multiple membrane-spanning helices that enable facilitated urea diffusion without energy expenditure. The SLC14A1 antibody allows visualization of this transporter in tissue sections and cell membranes, offering insight into how urea flux contributes to osmoregulation and urinary concentration.
Genetic polymorphisms in SLC14A1 have been linked to differences in erythrocyte antigen systems (the Kidd blood group, JK), as well as altered susceptibility to hemolysis and bladder cancer. The SLC14A1 antibody aids in detecting protein variants and assessing their expression patterns. In the kidney, Urea transporter 1 cooperates with aquaporins to control medullary osmolarity, optimizing urine concentration during water deprivation. Studies using this antibody have clarified how transporter regulation by hormones such as vasopressin adjusts urea recycling to maintain fluid balance.
Beyond its renal role, Urea transporter 1 participates in nitrogen balance and tissue detoxification. In erythrocytes, it ensures rapid equilibration of plasma and cytosolic urea concentrations, preventing osmotic stress. The SLC14A1 antibody is a key reagent for investigating how transporter dysfunction affects blood cell stability, renal concentrating ability, and urinary tract physiology. It also helps characterize SLC14A1 expression changes associated with metabolic disorders, dehydration, and cancer.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
