| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human VGLUT2/SLC17A6 recombinant protein (Position: M1-K561) was used as the immunogen for the SLC17A6 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SLC17A6 Antibody / VGLUT2 / Vesicular glutamate transporter 2 is a anti-SLC17A6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SLC17A6
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
SLC17A6 is encoded by the SLC17A6 gene located on human chromosome 11p14. The protein is approximately 582 amino acids long and belongs to the solute carrier 17 family of sodium-dependent phosphate transporters. SLC17A6 localizes to presynaptic vesicles in excitatory neurons, where it uses a proton electrochemical gradient to drive the uptake of glutamate into vesicles prior to release. Its activity directly influences synaptic strength and excitatory signaling fidelity.
An SLC17A6 antibody detects a 60 kilodalton band by western blot and reveals punctate presynaptic staining in neurons and neuropil under immunofluorescence. SLC17A6 expression is region-specific, being highly enriched in subcortical brain regions such as the thalamus, brainstem, and hypothalamus. This distribution distinguishes it from the related vesicular glutamate transporters SLC17A7 (VGLUT1) and SLC17A8 (VGLUT3), which have complementary roles in different neuronal populations.
Functionally, SLC17A6 controls the rate of glutamate release and synaptic efficacy. Knockout studies demonstrate that loss of SLC17A6 leads to severe impairment in excitatory neurotransmission, altered locomotor behavior, and perinatal lethality. Conversely, increased expression of SLC17A6 enhances glutamatergic tone, contributing to excitotoxicity in neurodegenerative disorders such as Parkinson's and Alzheimer's disease.
Beyond its neuronal function, SLC17A6 is expressed in certain endocrine tissues, where it contributes to glutamate-dependent signaling. Because of its precise localization and critical role in neurotransmitter loading, SLC17A6 serves as a robust marker for excitatory synapses.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
