SLC25A12 Antibody / ARALAR1

SLC25A12 antibody detects Calcium binding mitochondrial carrier protein Aralar2, encoded by the SLC25A12 gene. This protein belongs to the solute carrier family 25, which consists of mitochondrial carriers responsible for transporting metabolites across the inner mitochondrial membrane. Aralar2 functions primarily as an aspartate glutamate carrier, playing a critical role in the malate aspartate shuttle, which transfers reducing equivalents from the cytosol into the mitochondria. SLC25A12 antibody provides researchers with a reliable reagent for studying mitochondrial metabolism, neurodevelopment, and energy regulation.

Calcium binding mitochondrial carrier protein Aralar2 is highly expressed in the brain, heart, and skeletal muscle, reflecting its role in tissues with high energy demand. The protein's activity is regulated by calcium ions, which bind to EF hand domains in its N terminal region. When detected with SLC25A12 antibody, Aralar2 has been shown to facilitate the exchange of cytosolic glutamate for mitochondrial aspartate, linking amino acid metabolism with cellular energy pathways. This shuttle activity supports processes such as oxidative phosphorylation, urea cycle function, and neurotransmitter synthesis.

Mutations in SLC25A12 have been associated with neurodevelopmental disorders, including a subtype of early infantile epileptic encephalopathy. Patients with pathogenic variants present with seizures, developmental delay, and structural brain abnormalities. Studies using SLC25A12 antibody have confirmed that loss of Aralar2 disrupts neuronal energy metabolism and neurotransmitter balance. Beyond rare genetic disease, altered expression of this protein has been implicated in autism spectrum disorders, highlighting its importance in brain development and synaptic signaling.

Research has also connected Aralar2 to broader metabolic functions, including regulation of insulin secretion and lipid metabolism. SLC25A12 antibody has been used to demonstrate its presence in pancreatic islets, where glutamate aspartate exchange influences insulin granule release. In liver and muscle, changes in Aralar2 expression affect metabolic flexibility, making it relevant to diabetes and obesity studies. These findings highlight the wide physiological significance of Calcium binding mitochondrial carrier protein Aralar2 beyond the nervous system.

SLC25A12 antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting confirms expression in brain and muscle lysates, while immunohistochemistry demonstrates its localization to mitochondria within neuronal tissue. Immunofluorescence assays further confirm subcellular localization, colocalizing Aralar2 with mitochondrial markers. Functional studies using SLC25A12 antibody provide insights into how altered transporter activity affects metabolic pathways under physiological and pathological conditions.

By providing validated SLC25A12 antibody reagents,