| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human SLC27A4 was used as the immunogen for the SLC27A4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SLC27A4 Antibody / FATP4 / Solute carrier family 27 member 4 is a anti-SLC27A4 Rabbit antibody Recombinant Rabbit Monoclonal clone 30S71 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP) with listed reactivity in Human.
Key elements and design rationale
- Target: SLC27A4
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30S71, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF, IP
Biological background
Functionally, FATP4 antibody recognizes a 643-amino-acid multi-pass membrane protein localized to the endoplasmic reticulum and plasma membrane. SLC27A4 catalyzes both the import of exogenous fatty acids and their subsequent activation to acyl-CoA derivatives, which are required for lipid synthesis and beta-oxidation. Research using SLC27A4/FATP4 antibody has shown strong expression in skin keratinocytes and intestinal epithelia, where it supports lipid absorption and epidermal barrier integrity. This dual transporter-enzyme function ensures that fatty acids entering the cell are rapidly converted into metabolically usable forms.
Pathogenic variants in SLC27A4 (FATP4) cause ichthyosis prematurity syndrome, a rare autosomal recessive disorder marked by premature birth, respiratory distress, and defective skin barrier formation. Studies using FATP4 antibody demonstrate that loss of function disrupts epidermal lipid organization and compromises barrier cohesion, confirming the essential role of SLC27A4 in cutaneous and epithelial health.
Beyond congenital disease, altered SLC27A4/FATP4 expression has been implicated in obesity, diabetes, and cancer. Dysregulated fatty acid transport and activation can shift cellular energy balance, promoting insulin resistance and supporting tumor lipid demands. Research employing SLC27A4 antibody has linked elevated FATP4 activity to enhanced fatty acid uptake and metabolic reprogramming in malignant cells, highlighting its potential as a therapeutic target in metabolic and oncogenic pathways.
SLC27A4 antibody and FATP4 antibody reagents can be validated for western blotting, immunohistochemistry, and immunofluorescence to evaluate protein expression and localization. Western blotting confirms the ~72 kDa band expected for the full-length protein, immunohistochemistry highlights strong staining in skin and intestinal tissues, and immunofluorescence visualizes membrane-associated distribution. These complementary applications make the antibody a reliable marker for studying lipid transport and metabolic regulation.
By providing validated SLC27A4/FATP4 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
