| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human ZNT8/SLC30A recombinant protein (Position: M1-C361) was used as the immunogen for the SLC30A8 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SLC30A8 Antibody / ZNT8 / Zinc transporter 8 is a anti-SLC30A8 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: SLC30A8
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): IHC, IF, FACS, ELISA
Biological background
Structurally, ZNT8 is a 369-amino-acid multi-pass membrane protein of approximately 41 kilodaltons containing six transmembrane domains and a cytosolic C-terminus that mediates zinc ion transport driven by proton exchange. It localizes specifically to insulin granule membranes in pancreatic beta cells, where it sequesters zinc into vesicles, facilitating insulin hexamer formation and regulated exocytosis. ZNT8 expression is controlled by glucose and insulin signaling, reflecting its integration into metabolic feedback loops.
The SLC30A8 antibody is widely used in endocrinology, diabetes, and metabolism research to study zinc transport, insulin secretion, and beta-cell physiology. Western blot analysis detects a 41 kilodalton band corresponding to ZNT8, while immunofluorescence reveals granular cytoplasmic staining restricted to pancreatic islets. This antibody provides an excellent tool for exploring beta-cell biology and identifying autoantibody targets in diabetes pathogenesis.
Loss-of-function mutations in SLC30A8 reduce zinc transport activity and have been associated with altered diabetes risk. While some variants protect against type 2 diabetes by enhancing beta-cell resilience, others increase susceptibility by impairing insulin maturation and secretion. ZNT8 is also a major autoantigen in type 1 diabetes, with circulating autoantibodies serving as biomarkers for early disease detection. The SLC30A8 antibody enables detailed characterization of ZNT8 expression, localization, and immunogenicity in metabolic and autoimmune contexts.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
