| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human ZIP8/SLC39A8 recombinant protein (Position: E23-A438) was used as the immunogen for the SLC39A8 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SLC39A8 Antibody / ZIP8 / Solute carrier family 39 member 8 is a anti-SLC39A8 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, cell membrane.
Key elements and design rationale
- Target: SLC39A8
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
SLC39A8 is encoded by the SLC39A8 gene located on human chromosome 4q24. The protein is approximately 490 amino acids in length and belongs to the ZIP (Zrt/Irt-like protein) family of metal ion transporters. It localizes to the plasma membrane and mitochondria, where it facilitates cellular uptake of manganese, zinc, iron, and other cations necessary for enzymatic function and metabolic regulation.
The SLC39A8 antibody detects a 55 kilodalton band by western blot and reveals plasma membrane and cytoplasmic staining under immunofluorescence. Through regulation of manganese transport, SLC39A8 supports the activity of critical metalloenzymes such as mitochondrial superoxide dismutase and glycosyltransferases. Deficiency of SLC39A8 leads to systemic manganese depletion, mitochondrial dysfunction, and defective glycoprotein synthesis.
Genetic mutations in SLC39A8 are associated with congenital disorders of glycosylation, intellectual disability, and dystonia. The transporter also influences susceptibility to metabolic syndrome, hypertension, and schizophrenia through its impact on trace element homeostasis and inflammation. Overexpression or mislocalization of SLC39A8 alters cellular redox balance and zinc-dependent signaling pathways.
Because of its central role in metal transport and mitochondrial metabolism, SLC39A8 serves as a biomarker for trace element disorders and mitochondrial pathologies.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
