| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SLC6A16 recombinant protein (Position: E33-Y728) was used as the immunogen for the SLC6A16 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SLC6A16 Antibody / Solute carrier family 6 member 16 is a anti-SLC6A16 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Golgi, cell membrane.
Key elements and design rationale
- Target: SLC6A16
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
Structurally, SLC6A16 is a multi-pass transmembrane protein with twelve alpha-helical segments that span the plasma membrane. It contains conserved motifs responsible for sodium and chloride ion binding, which drive substrate transport against concentration gradients. SLC6A16 belongs to the neurotransmitter transporter family that includes serotonin (SERT), dopamine (DAT), and GABA (GAT) transporters, all sharing similar architecture and transport mechanisms. Co-localization studies show SLC6A16 localized to neuronal plasma membranes and synaptic vesicles, consistent with roles in neurotransmission and amino acid uptake.
Functionally, SLC6A16 participates in the reuptake and recycling of amino acids critical for neuronal homeostasis and synaptic activity. It maintains intracellular amino acid pools required for neurotransmitter synthesis and energy metabolism. In the testis, it may contribute to amino acid transport across Sertoli cell membranes, supporting germ cell development. The transporter couples substrate uptake to sodium and chloride gradients, allowing efficient nutrient capture even at low extracellular concentrations.
Dysregulation or mutation of SLC6A16 has been linked to neurodevelopmental and metabolic disorders. Variants have been associated with autism spectrum disorder, intellectual disability, and altered metabolic profiles in the brain. Altered expression in testicular tissues suggests additional roles in reproductive physiology. Pathway involvement includes neurotransmitter transport, amino acid metabolism, and sodium ion transmembrane transport. Expression of SLC6A16 increases during neuronal differentiation, indicating a developmental role in establishing amino acid homeostasis.
The SLC6A16 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
