| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A peptide specific to the SLC7A11 protein was used as the immunogen for the SLC7A11 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SLC7A11 Antibody / XCT / Solute carrier family 7 member 11 is a anti-SLC7A11 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, cell membrane.
Key elements and design rationale
- Target: SLC7A11
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ELISA
Biological background
SLC7A11 is encoded by the SLC7A11 gene located on human chromosome 4q28.3. The protein is approximately 55 kilodaltons and partners with the heavy chain subunit SLC3A2 (4F2hc) to form a functional heterodimeric transporter. This complex mediates sodium-independent cystine uptake coupled to glutamate export. Cystine is rapidly reduced intracellularly to cysteine, a rate-limiting precursor for glutathione synthesis, which protects cells from oxidative stress. SLC7A11 activity is therefore tightly linked to redox homeostasis and cellular antioxidant capacity.
The SLC7A11 antibody typically detects a 55 kilodalton protein by western blot, with strong expression in the plasma membrane and perinuclear compartments. It is transcriptionally regulated by NRF2 under oxidative stress and suppressed by p53 during stress-induced ferroptosis. Overexpression of SLC7A11 confers resistance to ferroptosis, a form of iron-dependent cell death caused by lipid peroxidation. Conversely, inhibition of SLC7A11 sensitizes cells to oxidative damage and promotes ferroptotic death, making it a key therapeutic target in oncology.
Functionally, SLC7A11 expression is elevated in various cancers, including lung, breast, and pancreatic tumors, where it supports tumor growth by enhancing glutathione synthesis and metabolic reprogramming. It is also implicated in neurodegenerative diseases and ischemic injury through its role in glutamate release and excitotoxicity.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
