| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SLC7A14 recombinant protein (Position: D9-E771) was used as the immunogen for the SLC7A14 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SLC7A14 Antibody / Solute carrier family 7 member 14 is a anti-SLC7A14 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Punctate cytoplasmic.
Key elements and design rationale
- Target: SLC7A14
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, FACS, ELISA
Biological background
Functionally, SLC7A14 antibody recognizes a multi-pass transmembrane protein localized to the lysosomal membrane, where it facilitates the uptake of arginine and other cationic amino acids from the cytoplasm into the lysosome. This transport is essential for maintaining amino acid balance, lysosomal integrity, and mTOR signaling. SLC7A14 is highly expressed in sensory neurons of the retina and cochlea, and loss of function leads to progressive vision and hearing loss. Mutations in SLC7A14 cause autosomal recessive retinitis pigmentosa and auditory neuropathy, making it a gene of growing clinical significance in neurodegeneration research.
At the molecular level, SLC7A14 contains 14 predicted transmembrane helices and shares structural features with other CAT family members but displays lysosome-specific trafficking signals. The transporter's localization depends on interactions with adaptor protein complexes such as AP-3 and lysosomal membrane proteins including LAMP1. SLC7A14 antibody is employed to study neuronal lysosome biology, amino acid metabolism, and neurodegenerative disease models. Studies demonstrate that reduced SLC7A14 impairs lysosomal function, increases oxidative stress, and triggers neuronal apoptosis. Expression analysis shows enrichment in inner hair cells, photoreceptors, and hippocampal neurons, linking it to sensory function and neural plasticity.
The SLC7A14 gene is located on chromosome 3q26.2 and encodes a 561-amino acid protein. Beyond sensory tissues, SLC7A14 transcripts have been detected in endocrine cells and fibroblasts, suggesting roles in nutrient sensing and metabolic regulation. Overexpression experiments indicate SLC7A14 contributes to amino acid-regulated mTORC1 activation, coupling lysosomal nutrient availability with cellular growth signals. The SLC7A14 antibody is valuable for immunofluorescence and confocal microscopy to confirm lysosomal localization and evaluate expression changes in disease models.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
