| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SLC7A7 recombinant protein (Position: M1-D498) was used as the immunogen for the SLC7A7 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SLC7A7 Antibody / Solute carrier family 7 member 7 is a anti-SLC7A7 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cell membrane.
Key elements and design rationale
- Target: SLC7A7
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ELISA
Biological background
Defects in SLC7A7 cause lysinuric protein intolerance (LPI), a rare inherited metabolic disorder characterized by impaired transport of cationic amino acids leading to protein intolerance, hyperammonemia, and growth retardation. In patients with LPI, mutations in SLC7A7 disrupt transporter assembly or function, resulting in abnormal accumulation of amino acids in urine and reduced absorption in the intestine and kidney. Research on SLC7A7 has advanced understanding of amino acid sensing pathways and their link to mTOR signaling, autophagy, and immune regulation.
At the cellular level, SLC7A7 is localized predominantly at the plasma membrane of polarized epithelial cells, where it supports nutrient absorption and nitrogen balance. The protein also contributes to macrophage activation and cytokine production, influencing inflammatory responses. Its expression is regulated by transcription factors including ATF4 and by nutrient availability, reflecting its central role in amino acid-responsive metabolic control. Experimental models indicate that SLC7A7 deficiency may lead to mitochondrial dysfunction and oxidative stress, providing insight into the metabolic complications of LPI.
Immunohistochemical analysis using SLC7A7 antibody shows staining in renal tubular epithelium, intestinal villi, and hepatocytes. It serves as a useful biomarker for investigating amino acid transporter function, metabolic disease mechanisms, and nutrient-dependent signaling. The SLC7A7 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
