| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SLC9A3 recombinant protein (Position: 667-831) was used as the immunogen for the SLC9A3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SLC9A3 Antibody / Sodium/hydrogen exchanger 3 is a anti-SLC9A3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA with listed reactivity in Human, Mouse.
Key elements and design rationale
- Target: SLC9A3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IP, ELISA
Biological background
SLC9A3 is encoded by the SLC9A3 gene located on human chromosome 5p15.33. The protein is approximately 834 amino acids long and belongs to the solute carrier 9 family of sodium/hydrogen exchangers (NHEs). It is predominantly expressed in the apical membrane of renal proximal tubule and intestinal epithelial cells, where it exchanges intracellular hydrogen ions for extracellular sodium ions, helping to regulate pH and volume.
The SLC9A3 antibody detects a 90 kilodalton band by western blot and shows apical membrane staining in epithelial tissues under immunofluorescence microscopy. Through its role in sodium absorption, SLC9A3 contributes to systemic blood pressure control and fluid balance. Regulation of SLC9A3 activity is mediated by phosphorylation and interaction with scaffold proteins such as NHERF1 and ezrin, linking it to hormonal control by angiotensin II and parathyroid hormone.
Mutations in SLC9A3 cause congenital secretory diarrhea and male infertility due to defective sodium transport and fluid absorption. In the kidney, SLC9A3 dysfunction results in impaired bicarbonate reabsorption and metabolic acidosis. Its regulation is critical for maintaining acid-base equilibrium, and its altered expression has been linked to cystic fibrosis and inflammatory bowel disease.
As an essential component of epithelial ion transport, SLC9A3 integrates metabolic, hormonal, and mechanical stimuli to maintain homeostasis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
