| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NHE8/SLC9A8 recombinant protein (Position: M1-Q568) was used as the immunogen for the SLC9A8 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SLC9A8 Antibody / Sodium/hydrogen exchanger 8 / NHE8 is a anti-SLC9A8 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SLC9A8
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, SLC9A8 antibody identifies a 576-amino-acid integral membrane protein localized primarily to the trans-Golgi network and apical plasma membrane. SLC9A8 contributes to luminal pH control in endosomes and the Golgi apparatus, influencing protein sorting and vesicular trafficking. It also maintains intestinal epithelial ion balance and supports mucosal barrier function.
The SLC9A8 gene is located on chromosome 20q13.13 and is widely expressed, with highest levels in kidney, intestine, and testis. NHE8 activity complements other sodium/hydrogen exchangers such as NHE3, particularly during development and epithelial differentiation. Its regulation ensures proper acid-base balance and cellular volume control.
Pathologically, loss of SLC9A8 function has been associated with intestinal dysfunction, renal tubular acidosis, and retinal degeneration. In epithelial cells, SLC9A8 deficiency impairs vesicular acidification and protein trafficking. Research using SLC9A8 antibody aids studies in membrane transport, epithelial physiology, and cellular pH regulation.
SLC9A8 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect sodium/hydrogen exchangers and intracellular transport proteins.
Structurally, Sodium/hydrogen exchanger 8 contains multiple transmembrane helices forming an ion translocation pathway and a cytoplasmic regulatory domain modulated by intracellular pH and signaling kinases. This antibody supports investigation of SLC9A8's function in vesicular trafficking and epithelial ion regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
