| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SLF2 recombinant protein (Position: E90-N1157) was used as the immunogen for the SLF2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SLF2 Antibody / SMC5-SMC6 complex localization factor protein 2 is a anti-SLF2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: SLF2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, ELISA
Biological background
SMC5-SMC6 complex localization factor 2 operates in coordination with its partner SLF1 to anchor the SMC5/6 complex at damaged replication forks. This localization facilitates homologous recombination repair and prevents collapse of stalled forks. The SLF2 antibody enables visualization of this critical repair factor in nuclear extracts and fixed cells, supporting detailed analysis of DNA repair kinetics and checkpoint activation. Its expression increases during replication stress and following exposure to DNA-damaging agents such as UV or ionizing radiation.
Disruption of SLF2 function compromises genomic integrity, leading to accumulation of DNA breaks, chromosomal abnormalities, and replication defects. Such genomic instability is a hallmark of cancer and aging. The SLF2 antibody aids in assessing how loss of SMC5-SMC6 recruitment affects DNA repair fidelity and cellular survival. In experimental models, depletion of SMC5-SMC6 complex localization factor 2 sensitizes cells to replication inhibitors, emphasizing its protective role during S phase.
Beyond replication stress response, SMC5-SMC6 complex localization factor 2 participates in maintaining telomere integrity and regulating chromatin compaction. The SLF2 antibody can be used to explore these broader nuclear processes, providing insight into how SMC5/6 and associated proteins orchestrate chromosomal architecture. Altered SLF2 expression has been implicated in tumorigenesis and therapy resistance, highlighting its relevance in cancer biology.
The SLF2 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing strong nuclear localization consistent with its DNA repair function.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
