| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SLFN12 recombinant protein (Position: E144-T578) was used as the immunogen for the SLFN12 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SLFN12 Antibody / Schlafen family member 12 is a anti-SLFN12 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Mouse, Rat.
Key elements and design rationale
- Target: SLFN12
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Structurally, SLFN12 is a 578-amino-acid protein of approximately 66 kilodaltons containing an N-terminal Schlafen domain and C-terminal helicase-like motifs that mediate RNA and ribosome binding. Unlike some other Schlafen family members, SLFN12 lacks DNA helicase activity but regulates translation elongation and protein turnover through non-catalytic interactions. It localizes mainly to the cytoplasm and perinuclear region, where it acts as a translational repressor for specific mRNAs involved in proliferation and stress response.
The SLFN12 antibody is widely used in immunology, virology, and epithelial biology research to study interferon-stimulated gene expression, translational regulation, and mucosal differentiation. Western blot analysis identifies a 66 kilodalton band corresponding to SLFN12, while immunofluorescence shows cytoplasmic and perinuclear staining in epithelial and immune cells. This antibody supports studies examining the intersection of innate immune signaling, protein translation, and epithelial homeostasis.
Functionally, SLFN12 inhibits translation of certain viral and cellular mRNAs, contributing to the antiviral defense mechanisms of interferon-stimulated cells. It also regulates epithelial differentiation in intestinal and airway epithelia by repressing translation of growth-promoting proteins and promoting barrier maturation. Dysregulation of SLFN12 expression is linked to abnormal epithelial remodeling, inflammation, and tumorigenesis. The SLFN12 antibody provides a high-quality tool for exploring Schlafen protein function, immune signaling, and translational control mechanisms.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
