| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SLIRP recombinant protein (Position: A11-F109) was used as the immunogen for the SLIRP antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SLIRP Antibody / SRA stem-loop interacting RNA-binding protein is a anti-SLIRP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Mitochondria (predominant), nuclear.
Key elements and design rationale
- Target: SLIRP
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ICC/IF, FACS, ELISA
Biological background
SLIRP is encoded by the SLIRP gene located on human chromosome 14q13.3. The protein is approximately 111 amino acids long and contains an RNA recognition motif responsible for binding SRA (steroid receptor RNA activator) and mitochondrial transcripts. SLIRP localizes primarily to the mitochondrial matrix but also associates with nuclear hormone receptors, linking energy metabolism with transcriptional regulation.
The SLIRP antibody detects a 12 kilodalton band by western blot and exhibits mitochondrial and nuclear staining under immunofluorescence. SLIRP forms complexes with LRPPRC, ensuring stabilization and translation of mitochondrial mRNAs encoding respiratory complex subunits. Loss of SLIRP leads to reduced mitochondrial translation, impaired oxidative phosphorylation, and decreased ATP production.
Beyond mitochondrial function, SLIRP interacts with steroid receptors such as estrogen receptor alpha and glucocorticoid receptor, modulating their transcriptional activity. By coupling nuclear hormone signaling with mitochondrial bioenergetics, SLIRP coordinates cellular responses to hormonal and metabolic cues. Dysregulation of SLIRP expression contributes to metabolic disorders, neurodegeneration, and certain cancers where mitochondrial dysfunction plays a role.
Because SLIRP integrates transcriptional control and mitochondrial energy metabolism, it represents a valuable target for understanding gene expression coordination and metabolic adaptation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
