| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SLX4 recombinant protein (Position: Q42-H1804) was used as the immunogen for the SLX4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SLX4 Antibody / Structure-specific endonuclease subunit SLX4 is a anti-SLX4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA with listed reactivity in Human. Reported localization: Nuclear.
Key elements and design rationale
- Target: SLX4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): IHC, IF, ICC/IF, ELISA
Biological background
SLX4 serves as a platform that organizes structure-specific nucleases into a regulated complex. It promotes timely incision and resolution of DNA structures to prevent recombination defects and chromosome missegregation. The SLX4 antibody is particularly useful for investigating DNA damage responses following genotoxic stress, allowing visualization of nuclear foci formation at replication and repair sites. By detecting SLX4 expression and localization, researchers can monitor its participation in maintaining DNA replication fidelity and chromosomal stability.
Mutations in SLX4 cause Fanconi anemia complementation group P (FANCP), a rare hereditary disorder characterized by genomic instability, bone marrow failure, and cancer predisposition. These pathogenic variants disrupt SLX4's ability to interact with partner nucleases, compromising crosslink repair. The SLX4 antibody supports studies of Fanconi anemia mechanisms and helps identify defects in DNA repair pathways that arise from SLX4 dysfunction. Analysis of Structure-specific endonuclease subunit SLX4 expression also provides insight into its regulatory interactions with checkpoint proteins such as ATR and FANCD2.
In addition to its nuclear repair functions, SLX4 has roles in telomere maintenance, homologous recombination, and mitotic checkpoint regulation. The SLX4 antibody enables mapping of these diverse activities by revealing subcellular localization dynamics during the cell cycle. Western blot and immunofluorescence experiments with this antibody show how SLX4 expression fluctuates in S-phase and peaks during mitosis, correlating with its role in resolving replication intermediates.
Because genomic stability is a cornerstone of cancer prevention, SLX4 has gained increasing relevance in oncology. Altered expression levels may influence tumor sensitivity to DNA-damaging agents such as cisplatin. The SLX4 antibody supplied by
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
