| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SMAP2 recombinant protein (Position: K142-K429) was used as the immunogen for the SMAP2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SMAP2 Antibody / Small ArfGAP 2 is a anti-SMAP2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: SMAP2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IP, FACS, ELISA
Biological background
Functionally, SMAP2 antibody identifies a 429-amino-acid protein containing an ArfGAP domain and a C-terminal clathrin-binding region. SMAP2 stimulates GTP hydrolysis on ARF1 and ARF6, facilitating vesicle uncoating and recycling. It plays a key role in synaptic vesicle turnover, receptor endocytosis, and intracellular trafficking, ensuring efficient communication between organelles.
The SMAP2 gene, located on chromosome 1p34.1, is widely expressed in brain, heart, and other tissues with active vesicular transport. In neurons, SMAP2 localizes to presynaptic terminals, where it interacts with clathrin and adaptor proteins to regulate synaptic vesicle cycling. Its activity coordinates endocytosis and exocytosis, maintaining synaptic plasticity and receptor availability.
Pathologically, aberrant SMAP2 expression or dysfunction may contribute to neurological disorders, including synaptic transmission defects and neurodevelopmental abnormalities. Its regulatory role in membrane dynamics also links it to cancer cell migration and secretion. Research with SMAP2 antibody provides insight into ARF signaling, vesicle recycling, and cellular communication mechanisms.
SMAP2 antibody is suitable for western blotting, immunocytochemistry, and immunoprecipitation to detect SMAP2 in cultured cells and tissues.
Structurally, SMAP2 contains an N-terminal ArfGAP catalytic domain that interacts with GTP-bound ARF, accelerating GTP hydrolysis, and a C-terminal region that binds clathrin and adaptor proteins. This modular structure allows SMAP2 to act as a molecular bridge between vesicle formation and signaling regulation. This antibody aids in understanding SMAP2�s contribution to intracellular trafficking and membrane remodeling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
