| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SMC1B recombinant protein (Position: R106-D1221) was used as the immunogen for the SMC1B antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SMC1B Antibody / Structural maintenance of chromosomes protein 1B is a anti-SMC1B Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: SMC1B
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, ELISA
Biological background
SMC1B forms heterodimers with SMC3 and associates with meiosis-specific cohesin components such as REC8, STAG3, and RAD21L. Together, these proteins establish and maintain cohesion between homologous chromosomes from prophase I until anaphase I, preventing premature separation. SMC1B also contributes to chromatin organization, double-strand break repair, and gene regulation during meiosis. Its expression is restricted mainly to germ cells in the testes and ovaries, though low levels may be detected in certain somatic tissues under specific developmental conditions.
The SMC1B antibody is used in cell biology, reproductive, and chromatin research to investigate cohesin complex formation and meiotic chromosome dynamics. Western blot analysis identifies a 145 kilodalton band corresponding to SMC1B, while immunofluorescence reveals chromosomal axis localization in meiotic cells. This antibody supports studies of meiotic cohesion, synaptonemal complex assembly, and chromosomal recombination.
Deficiency or mutation of SMC1B disrupts chromosome pairing and leads to infertility, meiotic arrest, or aneuploidy. Beyond meiosis, SMC1B may contribute to chromatin architecture and DNA repair in somatic cells. The SMC1B antibody provides a powerful tool for characterizing cohesin-mediated chromosome interactions and meiotic mechanisms.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
