| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | Recombinant full-length human SMNDC1 protein was used as the immunogen for the SMNDC1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SPF30 (survival of motor neuron-related-splicing factor 30), also known as SMNDC1 (survival motor neuron domain containing 1) or SMNR (SMN-related protein), is an essential splicing factor required for spliceosome assembly that belongs to the SMN family. It contains one Tudor domain with significant similarity to SMN (survival motor neuron) and is expressed in skeletal muscle, pancreas and heart, localizing to Cajal bodies and nuclear speckles. SPF30 plays an important role in spliceosome assembly and directly interacts with five U snRNPs. The loss of SPF30 causes spliceosome assembly to arrest at prespliceosomes (A complex). This supports a function for SPF30 in mediating the incorporation/recruitment of U4/U5/U6 tri-snRNP to the prespliceosome. In addition, the overexpression of SPF30 can lead to apoptosis.
This anti-SMNDC1 antibody is supplied as Purified (Mouse, Monoclonal (mouse origin), clone PCRP-SMNDC1-1A9, Mouse IgG2b, Unconjugated) and is designed to support common target-detection workflows after the on-page specifications.
Key elements and design rationale
- Target: SMNDC1
- Format: Purified
- Localization: Nucleus
- Species reactivity: Human
- Applications (listed): FACS, IF
- Conjugate: Unconjugated
- Clone and antibody class: Monoclonal (mouse origin), clone PCRP-SMNDC1-1A9, Mouse IgG2b
Because antibody performance can depend on epitope context, sample preparation, and biological state, interpret signals using appropriate controls and orthogonal evidence when possible.
Biological background
SMNDC1 is referenced in public gene/protein resources (e.g., UniProt and NCBI Gene), which provide curated names/synonyms, protein features, and pathway context. When designing assays, consider potential isoforms, post-translational modifications, and cell-type specific expression that may influence observed signal.
Research relevance and current trends
- Profiling SMNDC1 expression across model systems, perturbations, and time points to support mechanistic hypotheses.
- Combining antibody-based detection with multi-omics or imaging readouts to link SMNDC1 signal with phenotype.
- Using well-matched controls (isotype controls, genetic perturbations, or independent reagents) to strengthen interpretation of target-associated signal.
Common research applications
- FACS
- IF
Use the listed applications as a starting point and tailor experimental design to your sample type and readout requirements.
Notes for experimental interpretation
- Specificity considerations: closely related family members, isoforms, or PTMs can affect apparent specificity; confirm with independent approaches when critical.
- Controls: include negative controls and, when feasible, genetic or pharmacologic perturbations to support target attribution in your system.
- Species and sample context: differences in sequence, expression, fixation, or extraction conditions can change signal behavior across models.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
