| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SMTN recombinant protein (Position: E72-D881) was used as the immunogen for the SMTN antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SMTN Antibody / Smoothelin is a anti-SMTN Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Actin filaments), Nucleus.
Key elements and design rationale
- Target: SMTN
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, ELISA
Biological background
Smoothelin is tightly regulated during the phenotypic switch between contractile and synthetic smooth muscle states. During vascular injury or atherosclerotic changes, smooth muscle cells often downregulate SMTN expression as they transition to a proliferative phenotype, highlighting its role as a marker of mature, differentiated smooth muscle. Experimental models have shown that SMTN interacts with alpha-actinin, calponin, and other structural proteins to form an integrated cytoskeletal network essential for contractility. The gene is located on chromosome 22q12.3 and is transcriptionally regulated by serum response factor (SRF) and myocardin-related transcription factors, both central regulators of smooth muscle differentiation.
Immunohistochemical analysis reveals strong SMTN staining in vascular media, intestinal muscularis, and detrusor muscle, whereas non-muscle tissues lack expression. Because of its restricted distribution, SMTN antibody is widely used in smooth muscle cell research, vascular biology, and tumor pathology to distinguish contractile smooth muscle cells from fibroblasts or myofibroblasts. Loss or reduction of SMTN has been associated with vascular remodeling, hypertension, and certain myopathies. The smoothelin protein is also of interest as a potential biomarker for monitoring smooth muscle differentiation in stem cell-derived tissue engineering systems.
SMTN antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
