| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SMYD5 recombinant protein (Position: R18-F374) was used as the immunogen for the SMYD5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SMYD5 Antibody / SET and MYND domain-containing protein 5 is a anti-SMYD5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: SMYD5
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Structurally, SMYD5 is a 428-amino-acid protein of approximately 47 kilodaltons containing an N-terminal SET domain responsible for methyltransferase activity, a MYND-type zinc finger that mediates protein-protein interactions, and a C-terminal coiled-coil region that targets chromatin-associated complexes. It localizes primarily to the nucleus and interacts with components of the NCoR and SIN3A transcriptional corepressor complexes, linking histone methylation to large-scale transcriptional silencing.
The SMYD5 antibody is widely used in epigenetics, developmental biology, and immunology research to study histone methylation, chromatin regulation, and transcriptional repression. Western blot analysis detects a 47 kilodalton band corresponding to SMYD5, while immunofluorescence shows nuclear punctate staining consistent with chromatin localization. This antibody provides a reliable tool for investigating how methyltransferase activity contributes to transcriptional control and inflammatory gene regulation.
Functionally, SMYD5 acts as a negative regulator of inflammation by repressing toll-like receptor (TLR)-inducible genes and maintaining immune tolerance in macrophages. In embryonic development, SMYD5 influences the transcription of genes involved in metabolism and differentiation. Dysregulation of SMYD5 expression has been linked to metabolic disorders, neurodevelopmental abnormalities, and cancer, where altered histone methylation disturbs gene expression balance. The SMYD5 antibody supports mechanistic research into chromatin-based transcriptional regulation and methylation-dependent signaling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
