| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SNAP25 recombinant protein (Position: R31-L203) was used as the immunogen for the SNAP25 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SNAP25 Antibody / Synaptosomal-associated protein 25 is a anti-SNAP25 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SNAP25
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, WB, FACS
Biological background
SNAP25 is encoded by the SNAP25 gene located on human chromosome 20p12.2. The protein is approximately 25 kilodaltons and anchored to the plasma membrane via palmitoylated cysteine residues in its central linker region. SNAP25 interacts with syntaxin and synaptobrevin (VAMP) to form the ternary SNARE complex, which brings vesicle and plasma membranes into close proximity for fusion. This complex enables rapid and precise neurotransmitter release at presynaptic terminals.
The SNAP25 antibody detects a 25 kilodalton band by western blot and shows punctate presynaptic staining in neurons and neuroendocrine cells. SNAP25 participates in multiple exocytic events, including insulin secretion, catecholamine release, and synaptic vesicle recycling. It also regulates short-term synaptic plasticity by modulating vesicle pool dynamics.
Phosphorylation of SNAP25 by protein kinases such as PKC and CDK5 fine-tunes its role in vesicle priming and fusion probability. Mutations or altered expression of SNAP25 are linked to neurological and psychiatric disorders, including attention deficit hyperactivity disorder, epilepsy, and schizophrenia. Reduced SNAP25 function leads to synaptic transmission defects and cognitive impairment.
In addition to neurons, SNAP25 expression occurs in pancreatic beta-cells and endocrine tissues, reflecting its conserved role in vesicle exocytosis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
