| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse SNCB was used as the immunogen for the SNCB antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SNCB Antibody / Beta Synuclein is a anti-SNCB Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF) with listed reactivity in Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: SNCB
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF
Biological background
Beta-synuclein is encoded by the SNCB gene located on human chromosome 5q35.2. The protein is approximately 134 amino acids in length and intrinsically disordered, allowing it to adopt multiple conformations upon membrane binding. Unlike alpha-synuclein, Beta-synuclein lacks the hydrophobic non-amyloid component domain that promotes aggregation, and therefore is considered non-amyloidogenic under physiological conditions. This makes it an important natural modulator of synuclein aggregation balance.
The SNCB antibody detects a 14-20 kilodalton protein by western blot and shows intense staining in synaptic regions under immunohistochemistry. Beta-synuclein associates with synaptic vesicles through lipid interactions and regulates neurotransmitter release by modulating SNARE complex dynamics. It may act as a molecular chaperone preventing pathological alpha-synuclein aggregation. Reduced expression or mutations in SNCB have been linked to dementia with Lewy bodies and Parkinson-like neurodegenerative disorders.
Beta-synuclein also influences calcium signaling, vesicle recycling, and synaptic stability. Its expression is developmentally regulated, increasing as neurons mature. Under oxidative stress or protein misfolding conditions, Beta-synuclein may acquire toxic gain-of-function properties, forming soluble oligomers that disrupt vesicular trafficking. Recent evidence indicates that it may modulate dopaminergic neuron vulnerability through redox regulation and mitochondrial interactions.
Beyond neurodegeneration, Beta-synuclein is expressed in certain endocrine and peripheral tissues, where it regulates vesicle release. It has potential as a biomarker for neurological diseases and as a therapeutic target to stabilize alpha-synuclein homeostasis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
